Bacteria Identification Tools: Gram Negative Aerobic Non-fermentors

Probabilistic Identification of Bacteria: Gram Negative Aerobic Non-fermentors

Identify an unknown bacterial isolate by selecting the checkboxes for known test results, and clicking on the submit button. Leave boxes blank for unknown or indeterminate test results.

Test	Value
Motility at 37慢	Positive	Negative
Motility at RT	Positive	Negative
Growth at 37慢	Positive	Negative
Growth at RT	Positive	Negative
Pigment negative	Positive	Negative
Dirty pigment	Positive	Negative
Brown pigment	Positive	Negative
Violet pigment	Positive	Negative
Green pigment	Positive	Negative
Yellow pigment	Positive	Negative
Red pigment	Positive	Negative
Orange pigment	Positive	Negative
MacConkey growth	Positive	Negative
Catalase	Positive	Negative
Oxidase	Positive	Negative
Hugh & Leifson -ve or alkaline	Positive	Negative
H+L oxidative	Positive	Negative
H+L fermentative	Positive	Negative
H+L alkaline	Positive	Negative
Nitrate reduction	Positive	Negative
Simmon's citrate	Positive	Negative
Christensen's citrate	Positive	Negative
Urease	Positive	Negative
Gelatin stab	Positive	Negative
Gelatin plate	Positive	Negative
KCN	Positive	Negative
H2S paper	Positive	Negative
H2S TSI	Positive	Negative
Gluconate	Positive	Negative
Malonate	Positive	Negative
ONPG	Positive	Negative
PPA	Positive	Negative
Arginine dihydrolase	Positive	Negative
Lysine decarboxylase	Positive	Negative
Ornithine decarboxylase	Positive	Negative
PWS glucose acid	Positive	Negative
PWS glucose gas	Positive	Negative
0.4% Selenite	Positive	Negative
Casein hydrolysis	Positive	Negative
DNAase	Positive	Negative
Glucose ASS	Positive	Negative
Adonitol ASS	Positive	Negative
Arabinose ASS	Positive	Negative
Cellobiose ASS	Positive	Negative
Dulcitol ASS	Positive	Negative
Glycerol ASS	Positive	Negative
Inositol ASS	Positive	Negative
Lactose ASS	Positive	Negative
Maltose ASS	Positive	Negative
Mannitol ASS	Positive	Negative
Raffinose ASS	Positive	Negative
Rhamnose ASS	Positive	Negative
Salicin ASS	Positive	Negative
Sorbitol ASS	Positive	Negative
Sucrose ASS	Positive	Negative
Trehalose ASS	Positive	Negative
Xylose ASS	Positive	Negative
Ethanol ASS	Positive	Negative
Fructose ASS	Positive	Negative
Thornley Arginine	Positive	Negative
Tween 20	Positive	Negative
Tween 80	Positive	Negative
Tyrosine hydrolysis	Positive	Negative
Tyrosine pigment	Positive	Negative
Nitrite reduction	Positive	Negative
PHBA, growth	Positive	Negative
PHBA inclusions	Positive	Negative
Aesculin	Positive	Negative
Cetrimide	Positive	Negative
Fluorescence King's B	Positive	Negative
Growth 5慢	Positive	Negative
Growth 42慢	Positive	Negative
3-Ketolactose	Positive	Negative
Lecithinase	Positive	Negative
Starch hydrolysis	Positive	Negative
10% Glucose	Positive	Negative
10% Lactose	Positive	Negative
10% Bile, growth	Positive	Negative
40% Bile, growth	Positive	Negative
Bile stimulation	Positive	Negative
Haemolysis	Positive	Negative
4% NaCl, growth	Positive	Negative

Identification Results Table:

Enter test result data into checkboxes to proceed with identification

The following tests gave unexpected results for the most likely taxa:

No atypical results found

References:

Bryant, T.N. (2004) PIBWin -- software for probabilistic identification. Journal of Applied Microbiology 97(6):1326-7.

Holmes, B., Pinning, C.A. and Dawson, C.A. (1986) A probability matrix for the identification of Gram-negative, aerobic, non-fermentative bacteria that grow on nutrient agar. Journal of General Microbiology 132, 1827-1842