Probabilistic Identification of Bacteria: Gram Negative Aerobic Fermentative Rods

Identify an unknown bacterial isolate by selecting the checkboxes for known test results, and clicking on the submit button. Leave boxes blank for unknown or indeterminate test results.

Test Value
Motility at 37ºC Positive
Negative
Motility at RT Positive
Negative
Growth at 37ºC Positive
Negative
Growth at RT Positive
Negative
Pigment negative Positive
Negative
Dirty pigment Positive
Negative
Brown pigment Positive
Negative
Violet pigment Positive
Negative
Green pigment Positive
Negative
Yellow pigment Positive
Negative
Red pigment Positive
Negative
Orange pigment Positive
Negative
MacConkey growth Positive
Negative
Catalase Positive
Negative
Oxidase Positive
Negative
Hugh & Leifson -ve or alkaline Positive
Negative
H+L oxidative Positive
Negative
H+L fermentative Positive
Negative
H+L alkaline Positive
Negative
Nitrate reduction Positive
Negative
Simmon's citrate Positive
Negative
Christensen's citrate Positive
Negative
Urease Positive
Negative
Gelatin stab Positive
Negative
Gelatin plate Positive
Negative
KCN Positive
Negative
H2S paper Positive
Negative
H2S TSI Positive
Negative
Gluconate Positive
Negative
Malonate Positive
Negative
ONPG Positive
Negative
PPA Positive
Negative
Arginine dihydrolase Positive
Negative
Lysine decarboxylase Positive
Negative
Ornithine decarboxylase Positive
Negative
PWS glucose acid Positive
Negative
PWS glucose gas Positive
Negative
0.4% Selenite Positive
Negative
Caesin hydrolysis Positive
Negative
DNAase Positive
Negative
Adonitol PWS Positive
Negative
Arabinose PWS Positive
Negative
Cellobiose PWS Positive
Negative
Dulcitol PWS Positive
Negative
Glycerol PWS Positive
Negative
Inositol PWS Positive
Negative
Lactose PWS Positive
Negative
Maltose PWS Positive
Negative
Mannitol PWS Positive
Negative
Raffinose PWS Positive
Negative
Rhamnose PWS Positive
Negative
Salicin PWS Positive
Negative
Sorbitol PWS Positive
Negative
Sucrose PWS Positive
Negative
Trehalose PWS Positive
Negative
Xylose PWS Positive
Negative
Starch PWS Positive
Negative
Methyl red 37ºC Positive
Negative
Methyl red RT Positive
Negative
Voges-Proskauer 37ºC Positive
Negative
Voges-Proskauer RT Positive
Negative
Indole Positive
Negative
Acetate Positive
Negative
Mucate Positive
Negative
Growth at 5ºC Positive
Negative
Growth at 42ºC Positive
Negative

Identification Results Table:

Enter test result data into checkboxes to proceed with identification

The following tests gave unexpected results for the most likely taxa:

No atypical results found



References:

Bryant, T.N. (2004) PIBWin -- software for probabilistic identification. Journal of Applied Microbiology 97(6):1326-7.

Holmes, B., Dawson, C.A. and Pinning, C.A. (1986) A revised probability matrix for the identification of Gram-negative, aerobic, rod-shaped, fermentative bacteria. Journal of General Microbiology 132, 3113-3135.