Probabilistic Identification of Bacteria: Bacillus spp.

Identify an unknown bacterial isolate by selecting the checkboxes for known test results, and clicking on the submit button. Leave boxes blank for unknown or indeterminate test results.

Test Value
Length > 3µm Positive
Negative
Gram-positive Positive
Negative
Spores oval Positive
Negative
Spores central Positive
Negative
Spores bulging Positive
Negative
Aesculin hydrolysis Positive
Negative
Casein hydrolysis Positive
Negative
Hippurate hydrolysis Positive
Negative
Starch hydrolysis Positive
Negative
Urease Positive
Negative
Chloramphenicol resistance Positive
Negative
Nalidixic acid resistance Positive
Negative
Polymyxin resistance Positive
Negative
Streptomycin resistance Positive
Negative
Cellobiose acid Positive
Negative
Fructose acid Positive
Negative
Galactose acid Positive
Negative
Lactose acid Positive
Negative
Mannose acid Positive
Negative
Raffinose acid Positive
Negative
Salicin acid Positive
Negative
Xylose acid Positive
Negative
Citrate utilisation Positive
Negative
Succinnate utilisation Positive
Negative
Growth at 50C Positive
Negative
Growth in 10% NaCl Positive
Negative
Anaerobic growth Positive
Negative
Nitrate reduction Positive
Negative
Oxidase reaction Positive
Negative
Voges-Proskauer test Positive
Negative

Identification Results Table:

Enter test result data into checkboxes to proceed with identification

The following tests gave unexpected results for the most likely taxa:

No atypical results found



References:

Bryant, T.N. (2004) PIBWin -- software for probabilistic identification. Journal of Applied Microbiology 97(6):1326-7.

Priest, F.G. and Alexander, B. (1988) A frequency matrix for the probabilistic identification of some bacilli. Journal of General Microbiology 134, 3011-3018.