| Media - Mannitol Salt Agar For the Identification of Bacteria |
Mannitol Salt Agar: Click Here to View Images
Mannitol Salt Agar is used in the identification of S. auerus and other gram-
positive bacteria.
Growth Characteristics:
Mannitol salt agar is both a selective and differential agar. Mannitol salt
agar is inhibitory to most gram-negative bacteria, and is indicative of
mannitol fermentation. It is selective because it contains 7.5% sodium
chloride, which inhibits most bacteria, such as E. coli, from growing. It is
also differential, in that mannitol-fermenting bacteria, such as S.auerues
will turn the agar yellow, while bacteria that cannot ferment mannitol will not
produce a color change.
Typical Formulation per Liter:
Mannitol Salt Agar is used in the identification of S. auerus and other gram-
positive bacteria.
Growth Characteristics:
Mannitol salt agar is both a selective and differential agar. Mannitol salt
agar is inhibitory to most gram-negative bacteria, and is indicative of
mannitol fermentation. It is selective because it contains 7.5% sodium
chloride, which inhibits most bacteria, such as E. coli, from growing. It is
also differential, in that mannitol-fermenting bacteria, such as S.auerues
will turn the agar yellow, while bacteria that cannot ferment mannitol will not
produce a color change.
Typical Formulation per Liter:
- Proteose Peptone No. 3 10.0 g
- Beef Extract 1.0 g
- D-Mannitol 10.0 g
- Sodium Chloride 75.0 g
- Agar 15.0 g
- Phenol Red 0.025 g
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