Media - Cetrimide Agar
For the Identification of Bacteria
Cetrimide Agar:     Click Here to View Images

Cetrimide Agar is used in the identification of P. aeruginosa.

Growth Characteristics:
Cetrimide agar is both a selective and differential agar. Cetrimide agar is
inhibitory to most microbial flora unrelated to
P. aeruginosa, and is
indicative of pyocyanin, a green pigment produced by
P. aeruginosa. It is
selective because it contains cetrimide, a quaternary ammonia compound,
which inhibits  most other bacteria from growing. It is also differential, in
that  pyocyanin production is stimulated by the magnesium chloride and
potassium sulfate in the medium.
P. aeruginosa produces bright green
colonies on this agar.

Typical Formulation per Liter:

  • Pancreatic Digest of Gelatin                20.0 g
  • Magnesium Chloride                              1.4 g
  • Potassium Sulfate                                 10.0 g
  • Cetrimide                                                   0.3 g
  • Agar                                                          13.6 g
  • Glycerol                                                    10.0 mL